Nombre: ADRIANO FORTES MAIA
Fecha de publicación: 12/11/2020
Supervisor:
Nombre |
Papel |
|---|---|
| DANILO SALES BOCALINI | Advisor * |
Junta de examinadores:
| Nombre |
Papel |
|---|---|
| DANILO SALES BOCALINI | Advisor * |
| EDSON CASTARDELI | External Examiner * |
| FABIAN TADEU DO AMARAL | External Examiner * |
| LUCAS GUIMARÃES FERREIRA | Internal Examiner * |
| RODRIGO LUIZ VANCINI | Internal Examiner * |
Sumario: This study aimed to investigate the effects of physical exercise on behavioral and neurochemical parameters in rats exposed to cigarette smoke. For this purpose, male wistar rats (180-230g) were distributed in groups: Sedentary without exposure to cigarette smoke (-CS Sedentary), Exercise without exposure to cigarette smoke (-CS Exercise), Sedentary with exposure to cigarette smoke (+CS Sedentary) and Exercise with exposure to cigarette smoke (+CS Exercise). Exposure to cigarette smoke (60 min) was done for 60 consecutive days, 2 times a day, 7 days a week and swimming exercise (60 min) was done for 8 weeks, 5 days a week. For behavior evaluation, the rats were tested in the open field test (OFT) and object recognition test (ORT). We used as markers of oxidative stress in the rat hippocampi the measurements of the total superoxide-dismutase (SOD), cytosolic (CuZnSOD) and mithocondrial (MnSOD) concentrations, catalase (CAT) and the concentrations of glutathione peptides, in reduced (GSH) and oxidized (GSSG) forms. The levels of brain-derived neurotrophic factor (BDNF), kinase receptor tropomyosin (TrkB), synapsin, Pos-Sinaptic Density 95 and intracellular signaling proteins linked to neurogenesis, synaptic plasticity, inflammation and cell survival/death (CREB, AKT, P70S6k, p38, ERK 1/2, JNK, NF-KB, STAT3 and STA5) were measured in samples of hypocampal formation. The group exercised only had a longer time of locomotor activity compared to the other groups. The groups that performed the exercise (-CS exercise and +CS exercise) had a better performance in the exploratory activities of object recognition. There was a significant elevation in the levels of the different forms of superoxide-dismutase and catalase, as well as in the glutathione of the exercised animals exposed to cigarette smoke, however without affecting the GSH/GSSG ratio. The exercise was fundamental for the elevation of the levels of BDNF and TrkB in the rat hippocampus and altered the p38, STAT3 and STAT5 cellular signaling proteins. In conclusion, our findings indicate that the beneficial effects of exercise on motor, exploratory and memory activity may be partly related to changes in the BDNF-TrkB hippocampus proteins. We also observed that physical exercise intensifies changes in oxidative stress parameters in the formation of hippocampal in animals exposed to cigarette smoke.
Keywords: Exercise; Cigarette smoke; Hippocampus; BDNF; Oxidative stress.
